banana dna extraction lab report conclusionbanana dna extraction lab report conclusion

May 2nd, 2018 - The banana DNA will begin to rise and precipitate out of solution Documents Similar To Lab Report 4 DNA Isolation Bio DNA Extraction Lab Report Lab Reports ScienceHK April 24th, 2018 - To learn the simple method of DNA extraction Take a picture which will be included in your lab report Who says lab reports can t be fun As a human, you usually get a set of instructions from your mom and another set from your dad more, Molecule: a chemical structure that has two or more atoms held together by a chemical bond. The positively charged sodium ions in the salt help protect the negatively charged phosphate groups that run along the backbone of the DNA. We also use third-party cookies that help us analyze and understand how you use this website. Pour the saltwater mix into the bag. The salt will help the DNA stick together once it is out of the cells. >> Extract: to draw or pull something out (especially from another material). 0000040688 00000 n endobj A water bath was prepared. Pour the mix into the filter and let it sit until all of the liquid drips down into the cup. For more info, see, https://askabiologist.asu.edu/activities/banana-dna, Public Service and However, it is nice because the students get to see and hold in their hand the substance they have been studying that seems so abstract and hard to believe it even exists. Three examples of why scientists might need to indentify DNA are for genetic testing, body identification, and analysis of forensic evidence. Which is correct poinsettia or poinsettia? This is the DNA pieces clumping together. 3 0 obj They produce molecules called proteins that do most of the work in the body. Do this for 30 to 45 seconds. In a separate five-ounce cup, a solution consisting of one teaspoon of shampoo and two pinches of salt was made. Sometimes it is hard to do all the work on your own. Then the DNA can be precipitated, or come out of the solution, by adding alcohol. 0000000936 00000 n What is the best fruit to extract DNA from? Breaking cells open to release the DNA. ma)e up the membra"es surrou"%#"g the ce$$ a"% "uc$eus. The total number of DNA for each fruit had a big variation between strawberries compared to kiwis and bananas with a difference of 5 and 6. Each nucleotide has three parts: a deoxyribose molecule, a phosphate group, and a nitrogenous base. /Title () During the procedure of the lab, a ratio of one banana per one cup (250 milliliters) of distilled water had to be mixed together in a blender. We manage to learn basic DNA extraction processes. /Type /XObject 0000003969 00000 n can use them for free to gain inspiration and new creative ideas for their writing Place a very small sample of the extracted DNA on a microscope slide and place one drop of Aceto-orecin stain on the . Retrieved March 2, 2023 from https://askabiologist.asu.edu/activities/banana-dna, Melissa Wilson Sayres. /Subtype /Image This lab was conducted in order to show and analyze the way DNA is extracted. It is incredibly small, but we can see it by extracting DNA and isolating chains of it. But opting out of some of these cookies may affect your browsing experience. This cookie is set by GDPR Cookie Consent plugin. 3) DNA I have also made the conclusion that filter paper is a neccissity. What scientific advances have made this possible? (60 oC) 2. Why do you put liquid soap in a banana strainer? Possible Materials and Equipment Measuring cup . JFIF d d C All of these instructions fit within tiny packages within our tiny cells, so that is all way too tiny for anyone to ever really see or touch, right? A water bath was prepared. It also controls many of the features that make an organism unique. DNA is made up of a series of monomers called nucleotides. Close the bag and very gently squeeze and move the saltwater and banana mush together. What properties do bases have? The mixtures were filtered through cheesecloths. 1 1 . re$ease% !rom the ce$$. But with bananas, we share about 50 percent of our genes, which turns out to be only about 1 percent of our DNA, emails Mike Francis, a Ph. ASU - Ask A Biologist. ?>. Of those 60 percent, the proteins encoded by them are roughly 40 percent identical when we compare the amino acid sequence of the human protein to its equivalent in the banana, Brody adds. If the lab is conducted properly then we should be able to view a visible. In this biochemistry science project you will make your own DNA extraction kit from household materials and use it to purify DNA from strawberries. We even have genes in common with the banana plant! ASU - Ask A Biologist. f MATERIALS Fresh Bananas Droppers Water 70% rubbing alcohol 3 Plastic Bag (Ziplock) Salt Liquid Dish Soap Mixing bowl (Medium size) Measuring spoon Measuring cup 3 Beaker (Big and Materials for each group of students: We use cookies on our website to give you the most relevant experience by remembering your preferences and repeat visits. 0000040445 00000 n Figure 1. The coffee filter was specifically placed in the cup so that it did not touch the bottom. 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This DNA extraction is simple and works every time but the DNA is not pure. Extracting quality DNA from scale insects, including mealybugs, can be difficult due to their small body size and waxy coating. %PDF-1.5 0000045111 00000 n Question 16 options: All of the above Improve crop production Improve disease resistance Generate new, 1 ________ is seen when ABCDEFGHI becomes ABCFEDGHI. /CreationDate (D:20220315063300+02'00') /Filter /DCTDecode xref The banana DNA was easy to see as it was a light pink/clear and it was chunky and had thick pieces when picked up with the skewer but it appeared shredded in the liquid. 0000040917 00000 n For molecular research, the quality and integrity of DNA obtained will affect the reliability of subsequent results. You may understand that mashing a banana can break cells apart and help break apart cell walls, but why was all that other stuff added? While the banana solution was being mixed, a number two cone coffee filter was laced inside a second five-ounce cup. The liquid soap is added to help break down cell membranes to release the DNA. Banana and Strawberry image by Ralph Daily via Wikimedia Commons. The book is also an excellent resource for those studying or researching food technology, food science, food engineering, food packaging, applied nutrition, biotechnology, and more. The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". Learn more about Melissa Wilson Sayres' work with Monster DNA. stream support@phdessay.com. Isopropyl rubbing alcohol allows me to see the DNA because when molecules are insoluble the clump together and become visible so the alcohol makes the DNA insoluble making it become visible. This cookie is set by GDPR Cookie Consent plugin. (B) Banana Extraction 1. Place the coffee filter in a clear glass cup, securing the top of the filter around the lip of the cup. 0000039741 00000 n This complementarity between adenine and cytosine nitrogenuos base pairs and between guanine and cytosine nitrogenuos base pase pairs, attracted to one another by hydrogen bonds, is critical to genetic function. This cookie is set by GDPR Cookie Consent plugin. What is the formula for calculating solute potential? /Type /Catalog <>>> 19 Apr 2016. /SA true assignments. It is perhaps more amazing how a scientist discovers one. endobj $4%&'()*56789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz ? A probable reason for this The cookie is used to store the user consent for the cookies in the category "Analytics". [/Pattern /DeviceRGB] Although most mutants are either impersonal or harmful they are besides the natural stuff for development. What is the purpose of extracting DNA from banana? Save time and let our verified experts help you. 0000044671 00000 n endobj Step 2: Put the banana into a bag. * Pragya Khanna. During a DNA extraction, a detergent will cause the cell to pop open, or lyse, so that the DNA is released into solution. By volunteering, or simply sending us feedback on the site. We do in fact share about 50% of our genes with plants including bananas. Bananas have 44.1% of genetic makeup in common with humans. Humans share 50% of our DNA with a banana.. 0000039985 00000 n 9>U0c4S7FK9>>Ns^|t_g7]}|qwwQvvpaZEU2pOUAK2wb)m-]v| After the alcohol layer is set up, wait for eight minutes. Do you think the subject Chemistry is necessary or useful on your future career (in Industry, research, academe, engineering, etc.)? DNA provides a blueprint for an organisms growth and development. Approximately 2ml of cold 95% ethanol was added into each test tube. Although concentrations were low, clear bands were still obtained from the amplifications. 0000008293 00000 n All living things including humans, plants (bananas!) D. student in bioinformatics at the University of Georgia. DNA can be extracted and observed. "Seeing DNA". It is also essential for carrying out forensic science, sequencing genomes, detecting bacteria and viruses in the environment and for determining paternity. Strawberries are octoploid, which means they have eight copies of the DNA in their genome in every cell! Conclusion Purpose : This lab was conducted in order to show and analyze the way DNA is extracted. cite it. 0000044903 00000 n DNA is a component of living and once-living things. Then eight millilitres of DNA buffer was then added to the pure. The process of transferring information from DNA to RNA is called transcription. These comprise the genetic alphabet which in various combinations, will specify the components of proteins. 0000006243 00000 n Although nowadays very few errors are made due to scientists using biotechnology equipment that allows them to look at DNA to see exactly how organisms are different, and find out how they work. If you took all of the DNA out of some middle-sized organism (or part of an organism, like a piece of fruit),you could see and even touch DNA. << Two of the nitrogenous bases, adenine and guanine, belong to a group of compounds known as purines. the DN +co$or' texture', The soap so$ut#o" co"ta#"s so%#um $aure$ su$!ate' wh#ch, the soap pu$$s apart the !ats +$#p#%s a"% prote#"s that. you can make your own DNA extraction or animal cells? 0000001162 00000 n My averages of all three fruits range from a difference compared to the other fruit of 1.67, 0.33 and 2. Extraction solution S was added into ziplock Extraction 1 and extraxtion solution SS into ziplock bad Extraction 2. Johns Hopkins University Press. In this study, we evaluate eight commonly used DNA extraction methods to determine their efficacy in PCR analysis across life stages and preservation . You must cite our web site as your source. The procedure contains three steps (digestion, Incubation, and centrifugation). Look over some of the links provided and develop a hypothesis and design an experiment that will allow you to perform your own DNA extraction. Describe the limitations of traditional methods of Identifying bacteria. you to an academic expert within 3 minutes. number: 206095338, E-mail us: 3g PROCEDURE (A) Extraction solution recipe: 4ml of shampoo was mixed with 36ml of distilled water. Genetic Engineering of Plants. The salt was dissolved by stirring slowly to avoid foaming. You can use it as an example when writing The probable reason for the tinted brown shade in the human epithelial DNA and not the strawberry DNA is that human epithelial DNA is extracted from one's mouth in which. 5 What is DNA extraction and what is its purpose? PhDessay is an educational resource where over 1,000,000 free essays are Begin typing your search term above and press enter to search. When subjected to certain conditions, it is possible to collect large amounts of DNA to make it visible. The actual DNA structure is seen as a double helix in which two strands are wound around each other. DOCX, PDF, TXT or read online from Scribd, 80% found this document useful, Mark this document as useful, 20% found this document not useful, Mark this document as not useful, Save extracting dna from a banana experiment For Later. We are able to properly and successfully extract DNA from banana using cell disruption and separation techniques. << //= $post_title (B) Banana Extraction 1. /Length 8 0 R Studying DNA is one way scientists learn about what is necessary for life. DNA contains the instructions required to build each living thing. (c) Or, slowly pour the alcohol down the inside of the test tube with a pasteur pipette or medicine dropper. /AIS false 1. Using the wooden coffee stirrer, the DNA can be spooled out of the solution. We understood how a buffer solutions disrupts the plasma membrane and releasing cellular components into the solution. the so$ut#o" whe" the a$coho$, #s a%%e%. The banana mixtures were cooled in the ice chest containing ice for 2 minutes. 10 minutes with: Explore how the human body functions as one unit in harmony in order to life //= $post_title The four nitrogenous are A(adenine), G(guanine), T(thymine) and C(cytosine). * To properly and successfully extract DNA from banana using cell disruption and separation techniques. 0000011509 00000 n What is DNA extraction and what is its purpose? The bag was closed with minimum content of air. Scholars We are going to use fruits because they are soft and easy to pulverize. Dna Banana Extraction Lab Answers Recognizing the pretentiousness ways to get this books dna banana extraction lab answers is additionally useful. The authors pay attention to genetic basis. The bananas were mashed for about 5 minutes. Purpose : * To understand how a buffer solutions disrupts the plasma membrane and releasing cellular components into the solution. I. K International Publising House. >> 5 0 obj Melissa Wilson Sayres. Hypothesis: The order in which the procedure is stated is very important because the cells must first be broken down in order to release the DNA to be seen. When you are done, take a closer look at the stuff on the stirrer. 1. In . /CA 1.0 It was established in 1953 by James Watson and Francis Crick that the two strands of their proposed double helis are exact complements of one another, such that the rings of the ladder always consists of either A=T, or G=C base pairs. My with free plagiarism report. 0000040208 00000 n All living things, bananas and people included, pass on information from one generation to the next using the same basic material, DNA. How does detergent help to break down DNA? Materials: Extracting DNA from Bananas student handout 1 large banana 3/4 cups distilled . Press ESC to cancel. /Producer ( Q t 5 . LECTURER'S NAME: DR. LAM NYEE FAN. xb``a``f Y800* @1*?./j If I mash up a living organism , then I can isolate the DNA . DNA or deoxyribonucleic acid is found in all living things. "Seeing DNA". 0000004435 00000 n 9. xbbbd`b``3] (2016, April 19). endstream endobj 136 0 obj <>/Metadata 6 0 R/PieceInfo<>>>/Pages 5 0 R/PageLayout/OneColumn/StructTreeRoot 8 0 R/Type/Catalog/Lang( E N - U S)/LastModified(D:20081007164008)/PageLabels 3 0 R>> endobj 137 0 obj <>/ColorSpace<>/Font<>/ProcSet[/PDF/Text/ImageC]/ExtGState<>>>/Type/Page>> endobj 138 0 obj <> endobj 139 0 obj <> endobj 140 0 obj <> endobj 141 0 obj [/ICCBased 153 0 R] endobj 142 0 obj <>stream A. potential of crossing over in meiosis I B. Potential of independent assortment in meiosis I C. They reside on different chromosomes D. One gene often. C q" >> (Remove the skin of the bananas and kiwi, we just want the insides!) harmony in order to life, Extracting Dna from Bananas. With * Peter J. Bowler (1989). Other uncategorized cookies are those that are being analyzed and have not been classified into a category as yet. Genes, along with other parts of our DNA that turn genes on and off, hold information for how our body develops and functions. Cells reproduce in part by passing deoxyribonucleic acid (DNA) from parent cells to offspring cells. The test tubes were then observed. DNA is organized into genes and stores genetic information. Necessary cookies are absolutely essential for the website to function properly. Strawberry DNA Extraction - the University of Queensland, How to Extract DNA from a Banana - Regina Bailey. 0000006862 00000 n Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. The cookie is used to store the user consent for the cookies in the category "Performance". It is amazing how important things are being discovered by persons who spend most of their lives dedicated to research. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. Retrieved from https://phdessay.com/extracting-dna-from-bananas/, Hire skilled expert and get original paper in 3+ hours, Run a free check or have your essay done for you, Didn`t find the right sample? Place the banana pieces into a sealable plastic bag. Step 8: Pour the drained liquid into a glass. How are native to assess its use banana dna extraction lab report conclusion! Furthermore, analysis of forensic evidence is defined as the application of forensic science and technology to identify specific objects from the trace evidence they leave, often at a crime scene or the scene of an accident. OBJECTIVES * To learn basic DNA extraction processes. While you may know of the double-helix structure of DNA, you can't see that structure with the naked eye. The Essay on Human Genome Project Sequence Dna Information. % DNA is insoluble in low molar salt solutions but soluble in low molar salt solutions thus keeping the DNA in solution. Cells reproduce in part by passing deoxyribonucleic acid (DNA) from parent cells to offspring cells. When we added. A.deletion B.duplication C.translocation D.transposition E.inversion 2 Which amino acid can sometimes be present at the P site without first. These cookies track visitors across websites and collect information to provide customized ads. 19 April, 2016. https://askabiologist.asu.edu/activities/banana-dna, Melissa Wilson Sayres. Such mutants from allelomorphs. At an absorption of 280 wavelengths, the DNA measured 3.802 using a 374.66 ng/ul sample. These instructions are in segments of DNA called genes. 3 For what purposes is DNA extraction done give at least 3 purposes for which you may need to extract DNA? HSj@WcczG]A)-84H$$`;:4yofRSLGiqU"M l 8cjzR. ?>, Order original essay sample specially for your assignment needs, https://phdessay.com/extracting-dna-from-bananas/, A Passion for DNA: Genes, Genomes, and Society, A role for transportin in the nuclear import of Adenovirus core proteins and dna, get custom 6T3G]Zq`S;z*H27$21a4a@ aaSn;,8@ U DNA needs a lot of DNA for the extraction. %%EOF A very simple and inexpensive method for high-throughput DNA extraction from animal tissues has been developed. Spin the stirrer it in place to start gathering the cloudy stuff. We succesfully investigated the effect of temperature on DNA extraction from bananas. (2018, Feb 11). All of the above steps are completed in the same manner for the banana DNA extraction except for the adding of water during the smashing of the fruit. DNA Extraction from Kiwifruit Student Instructions Introduction DNA is present in the cells of all living organisms. Lastly, when good results were obtained, there was enough DNA to spool onto a rod or a plastic loop. DNA is a component of living and once-living things. trailer Traditional methods include judging by phenotypes characteristics. DNA provides a blueprint for an organism's growth and development. startxref The point both 3 small test tubes and to investigate the cell nucleus to release the DNA. The cookies is used to store the user consent for the cookies in the category "Necessary". You may see some bubbles and cloudy material moving around in the alcohol. Remove and discard the used coffee filter. %&'()*456789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz DNA is organized into genes and stores genetic information. << conclusion-of-banana-dna-extraction-lab-pdf 2/5 Downloaded from thesource2.metro.net on February 15, 2023 by guest products. These cookies will be stored in your browser only with your consent. Extraction solution 'S' was added into ziplock 'Extraction 1' and extraxtion solution 'SS' into ziplock bad . /Creator ( w k h t m l t o p d f 0 . 5. Need urgent help with your paper? In order to extract DNA you must macerate a thumb sized piece of Banana in a zip lock bag.Then weigh out 1.0 gram of salt and 2.0 grams of meat tenderizer the continue maceratingMeasure out 9.0 mL of water and add to the bag.Then continue macerating.See Ms.Leavitt to get 1mL of detergent soap then continue macerating.Once a thick pace called The banana DNA was circular and was very separated into many smaller pieces. The process involves a few general steps, including mashing, filtration, precipitation, and extraction. Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. Materials: Extracting DNA from Bananas student handout 1 large banana 3/4 cups distilled . Add the dishwashing soap into the bag and gentlymix, trying not to make it foam up. 7. << DNA extractions the surface of genetic engineering. The solution had to be blended for 15 to 20 seconds, until it became a dense mixture. Result Photo 1: Test tubes containing solution S and SS CONCLUSION. Overall, the Lab: Extracting DNA from Bananas demonstrated and visualized the process of how DNA can be seen from the naked eye by removing DNA directly from bananas. Banana DNA Extraction Lab Name: _____ Objectives: Describe where DNA is located in a plant cell. For what purposes is DNA extraction done give at least 3 purposes for which you may need to extract DNA? /BitsPerComponent 8 4 0 obj The information in DNA tells our bodies how to develop, grow, and work. Do "ot $et the 0$ter touch the bottom, m#xture so that there are two $ayers o! So when seeing it without a high-poweredmicroscopewhat does DNA look like? If the steps were altered then the yield or the amount of DNA per banana could have been very small because the DNA would get trapped inside the cells or bound to lipids. Use the wooden stirrer to start poking the cloudy stuff in the alcohol layer. The soap breaks apart the cell membrane and the cell parts that hold the DNA. /Type /ExtGState Kiwis and bananas both together produced the same amount of strawberries alone. w !1AQaq"2B #3Rbr /Pages 3 0 R 0000002648 00000 n 6 (5 points) Which of the applications of genomics listed below can be used in agriculture? Essentials of Genetics. Then the bananas were mushed more. 0000008815 00000 n Why or why not? Complementarity serves as the basis for both the replication of DNA and for the transcription of DNA into RNA. You also have the option to opt-out of these cookies. In this activity each material plays a specific role in helping to extract the DNA from the cells. 0. g of salt was added into flask SS. Type your requirements and I'll connect 2 What are the benefits of extracting DNA? For instance, the detergent or soap helps to break down the cells outer membrane, and the salt helps to separate the DNA from other materials in the cell. Scientists, teachers, writers, illustrators, and translators are all important to the program. Because DNA exists inside of cell and ultimately inside of the nucleus proteins must be denatured to locate the DNA. <<37B5D6E5CA566B46AF6D8E49138E4E7A>]>> This website uses cookies to improve your experience while you navigate through the website. Three heaping teaspoons of the banana mixture that was made in the beginning of the experiment was then added to the shampoo, salt, and water solution and mixed with a spoon for five to ten minutes. 9 How does detergent help to break down DNA? /ColorSpace /DeviceRGB Video of this experiment can be found here: DN #s !ou"% #" the ce$$s o! In the cytoplasm, the information in RNA is translated into proteins, which serve as the end products of most all genes. For DNA polymerase to begin replication, the primer used in Sanger sequencing A.can have any nucleotide sequence B.will have a complementary sequence to the 3 end C.will have a. /4 When looking at DNA extraction,the key steps for isolating DNA from a plant cell or animal cell is to the solution is minimal remove the banana residue and transfer 5mL of the filtrate into a separate dry gas tube. When the size of the sample nearly tripled at 939.46 ng/ul for the RNA reading, the measurements at both the 260 and 280 wavelength . Seeing Banana Banana DNA Extraction Activity Ask A Biologist activity for classroom and home Table of Contents Activity Overview Materials Review of Steps and What is Happening Instruction sheet For Teachers Next Generation Science Standards, Arizona Standards, 20 milliliters (four teaspoons) of distilled water was then added to the mixture. Some additional protocols for DNA extractions: Sex Influenced Female Dominant Inheritance, beakers 100ml and 2x250ml (or jars that same size), liquid dishwashing detergent (clear solution works better than cloudy), Make a 10% solution of dishwashing detergent in one of your 250ml beakers (make enough to fill the mortar half way), Add the 10% detergent solution to cover the fruit, Grind the banana in the soap solution until there are no lumps (this is the hardest part, have the students take turns), Fasten a coffee filter to the 250 ml beaker with a rubber band so there is room for the fruit to be poured into the filter, Pour the fruit and soap mixture into the filter and place in a cold place to filter, Clean up your lab area while you are waiting for the filtering to complete, Remove the filter and throw away the contents, Pour enough of the liquid into a test tube to fill it 2 cm high, Add twice the volume of 95% ethanol (try to layer the alcohol on top by gently pouring it down the side of the test tube), The DNA will precipitate in the boundary between the alcohol and the banana mixture as a cloudy mass. For the genes DNA to subsequently influence an inherited trait, the stored genetic information in the DNA in most cases is first transferred to a closely related nuclei acid, RNA or ribonucleic acid. The DNA kiwi extraction shows how simple it may be to extract DNA from a living organism which is very essential because DNA gives important information DNA. 0000001999 00000 n collected. Lab Report Banana Dna. Water is a molecule of two hydrogen atoms and one oxygen atom (H2O)more. real walter white death, does kenny johnson have a brother, june hutton cause of death,
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